天麻钩藤饮对自发性高血压大鼠血清Ca^(2+)浓度及血管平滑肌细胞钙通道的影响

Effect of Tian ma Gou teng Yin on serum Ca^(2+) and L-type Calcium Channels in the Vascular Smooth Muscle Cells in Spontaneously Hypertensive Rats(SHR)

目的:探讨天麻钩藤饮对自发性高血压大鼠(SHR)的血压及血管平滑肌细胞钙通道电生理特征的影响,阐明天麻钩藤饮对血压干预的作用机制。方法:选用12周龄自发性高血压雄性大鼠45只,随机分为5组。治疗4周后测量收缩压,用全自动图像分析系统进行分析,测定血清游离钙浓度,用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。结果:①天麻钩藤饮组用药4周后SHR血压下降最明显(P<0.01);②用药前后天麻钩藤饮组血清游离钙浓度改变没有统计学意义(P>0.05);③天麻钩藤饮组有明显减少血管平滑肌细胞Ica-L内流的作用。结论:①天麻钩藤饮和硝苯地平能够抑制早期的高血压,天麻钩藤饮长期降压效果优于硝苯地平;②天麻钩藤饮有明显的阻滞血管平滑肌细胞L型钙离子通道的作用,这可能是其降压作用机制之一。

Objective： To investigate the effect of Tian ma Gou teng Yin on blood pressure,the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells in SHR,to explain the mechanism of Tian Ma Gou Teng Yin to blood pressure.Methods： 12-week-old SHRs were assigned randomly into five groups.After 4-week-treatment,systolic blood pressure（SBP）,and serum free calcium concentration were measured,the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique（PCT）.Results： ①After 4-week-treatment,SBP was decreased in Tian ma Gou teng Yin group（P0.01）;②No significant difference of Tian ma Gou teng Yin was found in the serum free calcium concentration（P0.05）;③Tian ma Gou teng Yin can degradute the L-type calcium channels current of vascular smooth muscle cells.Conclusions： ①Both Tian ma Gou teng Yin and Nifedipine could repress early phase hypertension while no apparent statistical significance was found between them.The long-term antihypertensive effect of Tian ma Gou teng Yin is superior to Nifedipine;②Tian ma Gou teng Yin can block the L-type calcium channels current of vascular smooth muscle cells,which maybe one of the mechanisms of its antihypertensive function.