特应性皮炎患者外周血IL-4、IFN-、IL-2水平的检测

Detection of IL-4,IL-2 and IFN-γ levels in supernatant of PBMCs from patients with atopic dermatitis

目的了解特应性性皮炎(Atopic dermatitis,AD)患者外周血单个核细胞(PBMCs)培养上清液中部分细胞因子水平。方法分离培养97例AD患者及20例健康对照者外周血单个核细胞(PBMCs),采用ELISA方法检测细胞培养上清液白介素4(IL-4)、γ-干扰素(IFN-γ)、白介素2(IL-2)水平。结果 AD患者PBMCs培养上清液IL-4、IL-2水平高于健康对照组(P分别<0.01及0.05),而IFN-γ水平显著低于对照组(P<0.01)。结论细胞因子IL-4、IFN-γ、IL-2水平的变化在AD患者发病过程中起到一定的作用。

Objective To understand the levels of interleukin 4（IL-4）,interferon γ（IFN-γ） and interleukin 2（IL-2） in culture supernatants of peripheral blood mononuclear cells（PBMCs） from patients with atopic dermatitis（AD）.Methods The levels of IL-4、IFN-γ、IL-2 in supernatants of PBMCs from 97 patients with AD and 20 normal controls were measured by ELISA method.PBMCs were cultured for 72 hours in vitro.Results The levels of IL-4 and IL-2 were both significantly higher than those of the controls（P0.01 or 0.05）and levels of IFN-γ was significantly lower than that of in the controls.Conclusion The change of the levels of IL-4、IFN-γ、IL-2 played an important role in the pathogenesy atopic dermatitis.