八聚体转录因子4蛋白表达与非肌层浸润性膀胱癌的关系

Expression of Oct4 protein in non-musele-invasive bladder cancer

目的探讨八聚体转录因子4（Oct4）蛋白在非肌层浸润性膀胱癌中的表达情况，并分析其表达与患者临床病理特征及预后的关系。方法采用免疫组化生物素酶标法检测87例非肌层浸润性膀胱移行细胞癌和15例癌旁正常组织中Oct4蛋白的表达情况，并结合临床资料分析Oct4蛋白的表达在膀胱癌患者中的意义。结果正常组织中Oct4蛋白的表达水平明显低于肿瘤组织（P〈0．01）。G1级、G2级、G3级膀胱癌的Oct4蛋白阳性表达率分别为40．7％、69．4％、91．7％，差异有统计学意义（P〈0．01）。所有患者随访3—78个月，其中63例出现复发，复发组与未复发组中Oct4蛋白的阳性表达率分别为77．8％和37．5％，差异有统计学意义（P〈0．01）；21例复发患者出现进展，进展组与未进展组中Oct4蛋白的阳性表达率分别为71．4％和65．2％，差异无统计学意义（P〉0．05）。Oct4蛋白的表达与患者的年龄、性别、肿瘤的数目及大小无明显相关性（P〉0．05）。结论Oct4蛋白检测有助于早期发现膀胱肿瘤，并可用来判断肿瘤的恶性分化程度及检测患者术后的复发情况。

Objective To investigate the expression of Oct4 protein and analyze its correlation with the clinic pathological teatures and prognosis of non-muscle-invasive bladder cancer. Methods The oct4 protein expression was assessed by immunohistochemical analysis in 87 specimens of bladder transitional cell carcinoma and 15 specimens of adjacent normal tissues. A correlation between Oct4 and clinic pathological features was analyzed. Results The positive rate of Oct4 protein was significantly higher in bladder cancer than that in normal bladder tissue （P 〈 0.01 ）. The positive rate of Oct4 protein was 40.7% in G1 bladder cancer, 69.4% in G2 bladder cancer and 91.7% in G3 bladder cancer,and the differences was significant （P 〈0. 01 ）. All patients were followed up for 3-78 months, and 63 of them relapsed. The expression of Oct4 protein was significantly higher in patients of recurrence than in non-recurrence （77.8%：37.5%, P 〈0.01 ）. 21 patients of recurrence were in progression, and the expression of Oct4 protein had no significant differences between patients of progression and non-progression （71.4%： 65.2% ,P 〉0.05）. The positive rate of Oct4 protein was not related with gender, age, tumor number and size （P 〉 0.05）. Conclusion The detection of Oct4 protein is in favor of early detection of bladder tumor, estimation the degree of differentiation and surveillance for recurrence of superficial bladder cancer.