益生菌及早期进食对SD大鼠术后菌群失调的影响

EFFECTS OF PROBIOTICS AND EARLY EATING ON POSTOPERATIVE DYSBACTERIA IN RATS

目的探讨益生菌以及早期进食对SD大鼠术后肠道菌群失调的影响。方法将40只SD大鼠随机分为术后早期进食组、应用益生菌组、早期进食联合益生菌组及术后禁饮食组,每组10只。肠吻合术后分别采取以下处理措施:早期进食、应用益生菌、早期进食联合应用益生菌、禁饮食,共处理4天。第5天脱颈椎处死大鼠,无菌条件下取回盲部粪便进行细菌培养并计数。结果术后早期进食组、应用益生菌组以及早期进食联合益生菌组与术后禁饮食组比较,双歧杆菌及乳酸杆菌数量增加,而大肠杆菌数量减少,差异均有统计学意义(F=12.79～41.74,q=4.07～15.82,P<0.05)。早期进食联合益生菌组与术后早期进食组、应用益生菌组比较,双歧杆菌、乳酸杆菌数量增加,差异有统计学意义(q=5.21～7.91,P<0.05);大肠杆菌数量略减少,差异无统计学意义(P>0.05)。结论术后早期进食及应用益生菌有助于纠正SD大鼠术后肠道菌群失调。

Objective To investigate the influence of probiotics and early eating on postoperative intestinal dysbacteria in SD rats.Methods Forty SD rats were equally randomized to group A（early-eating group）,group B（probiotics group）,group C（combined early-eating and probiotics group） and group D（water-deprivation and fasting group）.After intestinal anastomosis,the following managements were offered to the rats in corresponding groups for four days： early eating;probiotics;combined early eating and probiotics;and no food and drink.At day 5,all the rats were killed and the stool in ileocecal junction was collected under aseptic technique for bacterial culture.Results Compared with group D,the number of bifidobacterium and lactobacillus in groups A,B,and C significantly increased,whereas the number of colibacillus decreased（F=12.79-41.74,q=4.07-15.82,P0.05）.Compared with groups A and B,the number of bifidobacterium and lactobacillus in group C increased,the difference being significant（q=5.21-7.91,P0.05）,and the number of colibacillus decreased a little（P0.05）.Conclusion Taking food early after surgery and using probiotics are conducive to correction of postoperative intestinal flora disequilibrium in SD rats.