宫颈癌组织STAT3和MMP-2表达及意义

EXPRESSIONS OF STAT3 AND MMP-2 IN CERVICAL CANCER

目的探讨信号转导和转录激活因子3（STAT3）与基质金属蛋白酶-2（MMP-2）在宫颈癌组织表达及意义。方法采用免疫组织化学SP法分别检测16例正常组织、50例宫颈上皮内瘤变（CIN）、50例宫颈癌组织中STAT3与MMP-2的表达水平。结果正常宫颈、CIN及宫颈癌组织中STAT3、MMP-2表达水平逐渐增高,各组间差异均有统计学意义（χ2=6.417~27.097,P〈0.05）。STAT3异常表达与宫颈癌的病理分级和临床分期及淋巴结转移有关（χ2=4.778~13.651,P〈0.05）;MMP-2的异常表达与临床分期及淋巴结转移有关（χ2=9.039、5.003,P〈0.05）,而与病理分级无关;两者与病人年龄、肿瘤大小及肿瘤类型均无相关性。宫颈癌组织STAT3与MMP-2的表达呈正相关（r=0.398,P〈0.05）。结论宫颈癌组织STAT3与MMP-2表达密切相关,两者表达水平可能与宫颈癌浸润转移有关,STAT3可能通过调控其下游靶基因MMP-2的表达影响宫颈癌的浸润转移。

Objective To investigate the expressions of signal transduction and activators of transcription-3（STAT3） and matrix metalloproteinase-2（MMP-2） in cervical cancer and their significance. Methods Immunohistochemical technique was used to detect expressions of STAT3 and MMP-2 in samples of 16 normal cervical tissue,50 cervical intraepithelial neoplasia（CIN） and 50 cervical cancer. Results The expressions of STAT3 and MMP-2 gradually increased in the order of normal cervical tissue,CIN,and cervical cancer,the differences between the three groups being statistically significant（χ2=6.417-27.097,P〈0.05）.The expression of STAT3 was related to clinical stage and pathological grade and lymph node metastasis（χ2=4.778-13.651,P〈0.05）;and that of MMP-2 was related to clinical stage and lymph node metastasis（χ2=9.039,5.003;P〈0.05）,while no relation to pathological grade.STAT3 and MMP-2 were positively correlated（r=0.398,P〈0.05）,but these two items were no correlation with patient＇s age,the size and type of the tumor. Conclusion In tissue of cervical cancer,the expression of STAT3 is closely related with that of MMP-2,both the expressions are probably associated with infiltration and metastasis of the cancer,the effect of STAT3 on the infiltration and metastasis of the cervical cancer may be through regulating the expression of its downstream target gene MMP-2.