摘要
目的探讨细胞特异性莫洛鼠白血病病毒插入位点-1（Bmi-1）和人端粒酶逆转录酶（hTERT）蛋白在胃癌组织表达及其与临床病理特征的关系,探讨二者在胃癌发生发展中的作用及其相关性。方法采用免疫组化PV-9000法分别检测70例胃癌、20例慢性萎缩性胃炎及10例癌旁正常胃黏膜组织标本中Bmi-1和hTERT蛋白的表达。结果胃癌组织Bmi-1和hTERT蛋白的阳性表达率分别为71.4%、78.6%,明显高于正常组织（0、0）及慢性萎缩性胃炎组织（20.0%、15.0%）,差异均有显著性（χ2=17.14~27.44,P〈0.01）。胃癌组织中Bmi-1和hTERT蛋白阳性表达均与癌组织的浸润深度、临床分期及有无淋巴结转移有关（χ2=6.375~26.480,P〈0.05）,而与病人的性别、Borrmann分型及分化程度等无关（χ2=0.047~1.052,P〉0.05）。胃癌组织中Bmi-1和hTERT蛋白的表达呈显著正相关（r=0.826,P〈0.01）。结论 Bmi-1、hTERT蛋白表达与胃癌的发生发展、浸润转移密切相关,可作为早期诊断胃癌及判断转移、预后的重要参考指标。
ObjectiveTo investigate the expressions of Bmi-1 and hTERT protein in gastric carcinoma,and explore their relation with clinical pathology.MethodsThe expressions of Bmi-1 and hTERT protein were detected,by PV-9000 immunohistochemical method,in specimens of 70 gastric carcinoma,20 chronic atrophic gastritis and 10 paraneoplastic normal gastric mucous membrane.ResultsThe positive expressive rates of Bmi-1 and hTERT in cancer were 71.4% and 78.6%,respectively,which were higher than that in normal tissue（0 and 0,respectively）,and that in chronic atrophic gastritis（20.0% and 15.0%,respectively）,all the differences were significant（χ2=17.14-27.44,P0.01）.The positive expressions of Bmi-1 and hTERT were closely correlated with the depth of cancer tissue infiltration,clinical stage and lymph node metastasis（χ2=6.375-26.480,P0.05）,and not with gender,Borrmann classification and differentiation（χ2=0.047-1.052,P0.05）.The expression of Bmi-1 in gastric cancer was positively correlated with hTERT（r=0.826,P0.01）.ConclusionThe expressions of Bmi-1 and hTERT protein are closely related with the occurrence,development,invasion and metastasis of gastric cancer,which can be used as an important reference for early diagnosis,metastasis judgment and prognostic prediction of this malignancy.
出处
《齐鲁医学杂志》
2011年第5期387-389,392,共4页
Medical Journal of Qilu
