氯吡格雷对支气管哮喘小鼠脑源性神经营养因子影响

EFFECTS OF CLOPIDOGREL ON BRAIN-DERIVED NEUROTROPHIC FACTOR IN MICE WITH BRONCHIAL ASTHMA

目的观察氯吡格雷对支气管哮喘小鼠血浆中脑源性神经营养因子(BDNF)的影响。方法雌性BALB/c小鼠共30只,随机分为阴性对照组(对照组)、哮喘模型组(模型组)和氯吡格雷干预组(干预组)。用酶联免疫吸附试验检测富血小板血浆中BDNF含量。结果对照组、模型组、干预组富血小板血浆BDNF水平分别为(6.11±2.34)、(26.67±7.59)(、6.28±2.63)μg/L,模型组较对照组增高,干预组较模型组降低,差异有显著性(F=28.62,q=11.34、10.56,P<0.01);对照组、模型组及干预组肺泡灌洗液嗜酸性粒细胞分别占(5.64±1.86)%(、24.62±2.66)%(、7.32±2.02)%,哮喘组较对照组增高,干预组较模型组降低,差异有显著性(F=25.14,q=10.39、9.62,P<0.01)。结论支气管哮喘小鼠血浆BDNF水平增高,经氯吡格雷干预后降低。

ObjectiveTo observe the effects of Clopidogrel on brain-derived neurotrophic factor（BDNF） in mice with bronchial asthma.MethodsThirty female BABL/c mice were evenly randomized to three groups： control,asthma-model,and Clopidogrel-intervention.Platelet-rich plasma BDNF was detected by enzyme linked immunosorbent assay（ELISA）.ResultsThe BDNF levels in control group,asthma-model group,and intervention group were（6.11±2.34）,（26.67±7.59）,and（6.28±2.63） μg/L,respectively;the level of BDNF in asthma-model group was higher than that in control group,and that in intervention group was lower than asthma-model group,the differences being significant（F=28.62;q=11.34,10.56;P0.01）.The counts of eosinophils in alveolar lavage fluid（ALF） in control,asthma-model and intervention groups accounted for（5.64±1.86）%,（24.62±2.66）%,and（7.32±2.02）%,respectively,that in asthma-model group was higher than that in the control,and that in the intervention was lower than that in the asthma-model,with significant differences（F=25.14;q=10.39,9.62;P0.01）.ConclusionThe level of BDNF in mice with bronchial asthma elevated,which was declined after intervention of Clopidogrel.