新疆马鹿角提取物对MC3T3-E1成骨细胞RANKL/OPG mRNA表达的影响

In-vitro Effects of the Extracts from the Xinjiang Cornu Cervi Elaphi on RANKL/OPG mRNA Expression in MC3T3-E1 Osteoblasts

目的研究新疆马鹿角提取物对MC3T3-E1成骨细胞核因子-kβ受体活化因子配体(RANKL)/护骨素(OPG)mRNA表达的影响。方法体外培养MC3T3-E1成骨细胞,分别加入含不同剂量新疆马鹿角提取物的培养液,72 h后分别提取细胞总RNA,用RT-PCR方法检测RANKL/OPG mRNA表达。结果培养72 h后RANKL/OPG mRNA表达的灰度值(0.312±0.0710),与对照组(2.017±0.1320)比较,新疆马鹿角提取物呈浓度依赖性降低RANKL mRNA的表达,增加OPG mRNA的表达,各剂量组RANKL/OPG mRNA吸光度比值降低。结论新疆马鹿角提取物可能通过调节成骨细胞RANKL/OPG的基因表达,而抑制破骨细胞介导的骨吸收。

Objective To study the effect of Xinjiang Cornu Cervi Elaphi extracts on RANKL/osteoprotegerin（OPG） mRNA expression in MC3T3-E1 osteoblasts.Methods MC3T3-E1 osteoblasts were cultured in vitro together with the different concentrations of Xinjiang cornu cervi elaphi extracts.Total RNA was extracted from MC3T3-E1 osteoblasts after culture for 72 h,and semi-quantitative reverse transcription PCR method was used to determine RANKL/OPG mRNA expression.Results The optical density（OD） of RANKL / OPG mRNA expression of Xinjiang Cornu Cervi Elaphi extracts was（0.312±0.0710） after 72 h,less than（2.017±0.1320） in the control group,indicating that the extracts inhibited the level of RANKL mRNA and promoted the level of OPG mRNA in a dose-dependent manner.The RANKL/OPG mRNA ratio in extract groups at different concentrations was also decreased.Conclusion Xinjiang Cornu Cervi Elaphi extracts may inhibit the osteoclastic bone resorption through regulating the expression of RANKL/OPG mRNA in MC3T3-E1 osteoblasts in vitro.