三七叶抗抑郁活性提取物PnGL中皂苷类成分分析

Saponin Components Analysis of Antidepressant Extract PnGL from Leaves of Panax Notoginseng

目的建立三七叶抗抑郁活性提取物PnGL中皂苷类成分含量测定的方法。方法采用香荚醛-高氯酸比色法,分别以人参二醇及人参皂苷Rb3为对照品,测定总皂苷的含量。采用HPLC法,Ultimate AQ-C18色谱柱,甲醇-水(69∶31)为流动相,检测波长203 nm,柱温为25℃,同时测定PnGL中主要成分人参皂苷Rb1、Rb2、Rc、Rb3的含量。结果以人参二醇及人参皂苷Rb3为对照品所测定的总皂苷含量,分别为97.25%、95.76%,差异不大。HPLC检测基线平稳,人参皂苷Rb1、Rb2、Rc、Rb3分离良好,分离度1.5以上,平均加样回收率分别为99.24%(RSD=1.63%)、96.41%(RSD=1.21%)、96.81%(RSD=0.86%)、100.16%(RSD=1.84%)。结论含量测定方法准确、重复性好,可为PnGL质量控制提供依据。

Objective To establish the determination method for saponin components of the antidepressant extract PnGL from leaves of Panax notoginseng（Burk.） F.H.Chen.Methods The contents of total saponins were determined by vanillin-perchloric acid colorimetric method with panaxadiol and ginsenoside Rb3 as standard substances.The contents of total ginsenosides Rb1,Rb2,Rc,and Rb3 were determined simultaneously by high performance liquid chromatography.The chromatographic conditions were as follows：Ultimate AQ-C18 analytical column,a mixture of methanol-water（69 ∶ 31） as the mobile phase,detection wavelength at 203 nm,and colunm temperature of 25 ℃.Results When panaxadiol and ginsenoside Rb3 were as the standard substances,the content of total saponins was 97.25 % and 95.76 % respectively,showing little difference.Ginsenoside Rb1,Rb2,Rc,and Rb3 had been well isolated by high performance liquid chromatography,and their resolutions were over 1.5 with the stable detection baseline.The average recovery rate was 99.24 %（RSD =1.63 %）,96.41 %（RSD=1.21 %）,96.81 %（RSD=0.86 %）and 100.16 %（RSD=1.84 %） for ginsenoside Rb1,Rb2,Rc and Rb3 respectively.Conclusion The method is reliable and reproducible,which provides a reference for the quality control of PnGL.