HPLC-ELSD法测定清肺抑火丸中知母皂苷BII的含量

Determination of Timosaponin BII in Qingfei Yihuo Pills by HPLC-ELSD

目的建立高效液相色谱-蒸发光散射检测器测定清肺抑火丸中知母皂苷BII含量的方法。方法采用Diamonsil-C8色谱柱,柱温为30℃;以乙腈-水(21∶79)为流动相,流速为1.0 mL.min-1;蒸发光散射检测器检测,漂移管温度为80℃,载气压力30 psi。结果在选定的色谱条件下,以知母皂苷BII峰面积的常用对数(Y)对浓度的常用对数(X)进行线性回归,回归方程为Y=1.7850X+1.9723,线性范围为8.2～82.0μg.mL-(1r=0.9997),知母皂苷BII的平均回收率为101.2%,RSD为1.05%(n=6)。结论该法可用于清肺抑火丸中知母皂苷BII的含量测定。

Objective To establish a HPLC-ELSD method for the determination of timosaponin BII in Qingfei Yihuo Pills.Methods Chromatography was performed on a Diamonsil-C8 at 30 ℃.The mobile phase consisted of acetonitrile-water（21 ∶ 79） with a flow rate of 1.0 mL·min^-1.An evaporate light-scattering detector（ELSD） was used as the detector with gas pressure of 30 psi and drift tube temperature of 80 ℃.Results The regressive equation was obtained by using logarithm of timosaponin BII peak area as Y and with logarithm of timosaponin BII content as X,and the equation was Y=1.7850X＋1.9723（r=0.9997）.The linear range of timosaponin BII was 8.2～82.0 g·mL-1.The calibration curves of timosaponin BII was linear in the range of 8.2～82.0 g·mL^-1,and the average recovery for timosaponin BII was 101.2 %,RSD was 1.05 %（n=6）.Conclusion The method can be used for quantitative determination of the preparation.