摘要
目的建立栝楼根胶囊的质量标准。方法采用薄层色谱法对栝楼根胶囊中的天花粉、黄连、知母、麦冬进行鉴别;采用高效液相色谱法对栝楼根胶囊中小檗碱进行含量测定。色谱柱为Zorbox SB C18柱(150 mm×4.6 mm,5μm);流动相为乙腈-0.05 mol/L磷酸二氢钾(22∶78);检测波长为350 nm;流速为1.0 mL/min;柱温为25℃。结果小檗碱在1.0～25μg/mL浓度范围内呈良好的线性关系,r=0.9998,小檗碱的平均回收率分别为98.26%,RSD为1.69%。结论本法操作简便、快速、准确,可用来控制栝楼根胶囊的质量。
Objective To establish the quality standard of Gualougen Capsules.Methods Radix Trichosanthis,Rhizoma Coptidis,Rhizoma Anemarrhenae and Radix Ophiopogonis were identified by TLC.The content of berberine was determined by HPLC.Zorbox SB C18（150 mm×4.6 mm,5 μm） was used as chromatographic column.Mobile phase consisted of acetonitrili-0.05 mol / L potassium dihydrogen phosphate（22 ∶ 78）.The detection wavelength was at 350 nm.The flow rate was 1.0 mL / min.and the temperature of column was set at 25 ℃.Results The result of TLC showed the herbal material was well separated without interference.The linearity of berberine was good in the range of 1.0～25 μg/mL.The average recovery of berberine was 98.26 %,and RSE=1.69 %.Conclusion The method is simple,reliable,and accurate,and can be applied as the quantity control method of Gualougen Capsules.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2011年第6期681-684,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
中匈国际合作项目(2008DFA31050)
