摘要
目的探讨血清转化生长因子-β1(TGF-β1)及相关促炎性细胞因子在糖尿病性牙龈炎发病机制中的作用。方法选择雄性SD大鼠30只,按随机数字表法分为正常对照组、牙龈炎组及糖尿病性牙龈炎组。应用酶联免疫吸附法(ELISA)检测牙龈炎、糖尿病性牙龈炎及健康大鼠牙龈组织TGF-β1、白细胞介素6(IL-6)及白细胞介素8(IL-8)的含量,免疫组化染色观察牙龈组织中TGF-β1表达水平的变化。结果与健康大鼠相比,牙龈炎组及糖尿病性牙龈炎组大鼠牙龈组织TGF-β1I、L-6及IL-8含量均明显升高,并有递增趋势。TGF-β1在健康大鼠牙龈组织中呈弱阳性反应,反应呈黄色,牙龈炎组及糖尿病性牙龈炎组TGF-β1表达较正常对照组明显增强,反应为棕黄色,呈强阳性改变。结论 TGF-β1在糖尿病性牙龈炎的发生、发展中起重要作用。
Objective To study the role of transforming growth factor β1 and correlated inflammatory factors in diabetic gingivitis.Methods 30 male Sprague-Dawley rats were divided in to 3 groups by table of random number：normal control group,gingivitis group,and diabetic gingivitis group.The concentrations of TGF-β1,IL-6 and IL-8 of periodontal tissue were measured by ELISA,and TGF-β1 expression in periodontal tissue of rat was observed by immunohistochemistry.Results The level of TGF-β1,IL-6 and IL-8 of gingivitis group and diabetic rat gingivitis tissues were significantly elevated than healthy rats.The expression of TGF-β1 in healthy rats was weakly positive.The expression of TGF-β1 in gingivitis group and diabetic group were significantly increased,showing strong positive reaction.Conclusion The expression of TGF-β1 plays a very important role in the onset and development of diabetic gingivitis.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第22期2191-2193,2196,共4页
Chongqing medicine
基金
重庆市卫生局课题(07-2-115)
关键词
牙周炎
转化生长因子Β1
糖尿病
periodontitis
transforming growth factor beta1
diabetes mellitus
