扩展莫尼茨绦虫膜联蛋白B3基因组织表达差异分析

Analysis of the Differential Expression of the PICKI Gene in Moniezia expansa

【目的】探索膜联蛋白B3(Annexins B3)基因在扩展莫尼茨绦虫生长发育中的作用。【方法】研究以beta-Tubulin基因作为内参基因,采用SYBR Green real-time RT-PCR方法检测该基因在扩展莫尼茨绦虫四个不同发育阶段即头节、幼节、成节、孕节中的表达差异。【结果】标准曲线分析显示,Annexins B3和beta-Tubulin基因的Ct值与阳性质粒的浓度均呈良好的线性关系,相关系数均大于0.991,溶解曲线分析表明,产物为特异的单峰,具有较高的特异性。【结论】膜联蛋白B3基因在虫体个发育阶段中的表达丰度存在差异,从高到低依次为孕节、头节、成节、幼节。

[ Objective ] The program aimed to explore the Annexins B3 gene in the development of Moniezia expansa. [ Method ] This study developed a SYBR green real- time fluorescent quantitative PCR assay for detection of Moniezia expansa mRNA with Moniezia expansa beta - Tubulin as an interna control to construct real -time fluorescent quantitative PCR assay. [ Result ] The results showed a good linear relationships （ 〉0. 991 ） between the Ct value and the concentration of positive plasmid for each gene. The melting curve analysis showed the product was specific to a single peak, with high specificity. [ Conclusion ] Real -time PCR results showed that the expression abundance of Anrtexins B3 gene was from high to low in gravid segment, scolex, mature segment and immature segment in order.