终末期肝病患者乙型肝炎病毒感染与肝祖细胞激活的关系

Study of HBV infection and hepatic progenitor cell activation in end-stage liver disease

目的探讨终末期肝病患者乙型肝炎病毒感染与祖细胞激活和扩增的关系。方法 16例终末期慢性乙型肝炎患者的肝活检标本,以细胞角蛋白(CK7)为标记做免疫组织化学,定量分析祖细胞数目和胆管反应面积的相关性。结果全部患者均从组织学证实为肝硬化,并且炎症反应严重(炎症活动度评分12～17分)。祖细胞数目和胆管反应面积呈显著正相关。血清HBVDNA水平与祖细胞数目和胆管反应面积均显著相关。结论在终末期慢性乙型肝炎患者中,祖细胞的激活和扩增明显,HBV感染可能参与肝祖细胞的激活和扩增。

Objective To study the activation of hepatic progenitor cells(HPCs) and its relationship with HBV infection in patients with end-stage chronic hepatitis B.Methods Sixteen cases of liver cirrhosis caused by chronic hepatitis B were studied.The numbers of HPCs and area of ductular reactions were quantitively analyzed on the cytokeratin 7(CK7)-stained sections.Results All of the sections showed liver cirrhosis and severe inflammation(HAI ranged from 12 to 17).The number of HPCs were correlated with the area of ductular reactions positively.Serum HBV DNA level was independently associated with HPCs activation and ductular reactions.Conclusions In end-stage of chronic hepatitis B,HBV infection may be involved in the activation of progenitor cells in certain environments.