荨麻疹患者外周血嗜碱性粒细胞表面CD63表达的研究

Analysis of CD63 expression on basophils from human peripheral blood of the patients with urticaria

目的观察荨麻疹患者外周血中活化的嗜碱性粒细胞表面表达CD63的数量,探讨嗜碱性粒细胞的活化程度,为临床检测提供新方法。方法用屋尘螨浸液作划破试验,筛选敏感的荨麻疹患者。用CD63-FITC等抗体标记嗜碱性粒细胞,流式细胞技术检测CD63的表达情况。结果急、慢性患者活化嗜碱性粒细胞百分率分别为33.77～15.75%、11.66～5.25%,与正常人比较均有显著差异(P<0.05);经变应原刺激后,急、慢性患者活化嗜碱性粒细胞百分率分别为60.25～18.88%、22.22～7.89%,急、慢性患者活化前、活化后也均存在明显差异(P<0.05),而且急性患者活化程度更高(P<0.05)。结论通过测定嗜碱性粒细胞表达CD63可能有助于过敏的诊断。

Objective To study the percentage of CD63 basophils of the patients with urticaria,and to offer immunological method for detecting allergen.Methods The patients with acute and chronic urticaria were screened for those sensitive to allergens by scratch test with Der P allergens.After being isolated from anti-clotting human peripheral blood with heparin,the basophils were stained with CD63-FITC.Finally the percentage of CD63 on stained cells was analyzed by Three-color immunofluorescence flow cytometry.Results The percentage of CD63 basophils of the patients with acute and chronic uticaria were 33.77～15.75%and 11.66～5.25%,and differences between them and those of normal group were significant(P<0.05).After Der P stimulation, the percentage of CD63 basophils of the patients with acute and chronic patients were 60.25～18.88%and 22.22～7.89%,the differences between them and those of no-stimulation were significant(P<0.05).Acute patients were more sensitive than chronic patients.Conclusion Analysis of CD63 expression on basophils may be helpful to detect allergen.