143例天疱疮、大疱性类天疱疮直接免疫荧光检查结果分析

Results analysis of direct immunofluorescence test of 143 cases with pemphigus and bullous pemphigoid

目的回顾性观察天疱疮、大疱性类天疱疮直接免疫荧光检查(DIF)结果,分析及评估其实验方法。方法活检取材,冰冻切片,DIF染色,荧光显微镜观察。结果免疫病理确诊143例,其中寻常型天疱疮(PV)阳性75例(52.4%)、红斑型天疱疮(PE)阳性13例(9.1%)、大疱性类天疱疮(BP)阳性53例(37.1%)、副肿瘤天疱疮(PNP)2例(1.4%)。临床与免疫病理诊断的符合率PV为65.3%(49/75),PE为61.5%(8/13),BP为69.8%(37/53),三者比较差异无统计学意义(P>0.05)。结论DIF对常见大疱性皮肤病的诊断是一项重要的、快速而特异性较高的检查方法,但仍需紧密结合临床确诊。

Objective To retrospectively analyze the results of direct immunofluorescence (DIF) test in 143 cases of pemphigus and bullous pemphigoid (BP). Methods Conducted biopsis routinely,prepared frozen sections and detected the antibodies by DIF test. Results Immunopathology diagnosis was established to every patient,among the total 143 cases,the number of pemphigus vulgaris (PV) was 75(52.4%),pemphigus erythematosus (PE) was 13(9.1%),BP was 53(37.1%) and paraneoplastic pemphigus was 2(1.4%). The accordance rate between clinical and the immunopathology diagnosis was 65.3% (49/75)in PV,69.8% (37/53) in BP,and 61.5% (8/13) in PE. There was no signifi cant difference between the groups(P>0.05). The types of the autoantibodies are similar to the results which had been reported. Conclusion DIF test is an important and fast diagnostic method with high specifi city in the diagnosis of bullous dermatosis. Meanwhile combinating with the cilinical manifestations is still important.