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二氮嗪对长时程低温保存移植鼠心ATP酶的影响及意义

Effects and influences of diazoxide on ATPase of long-term preservation heart in orthotopic cardio-transplantation of rats
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摘要 目的观察线粒体ATP敏感性钾离子通道开放剂二氮嗪(Diazoxide,DE)对移植鼠心心肌细胞膜Na+-K+-ATPase和肌浆网Ca2+-ATPase活性的影响,并探索DE处理后Celsior液对长时程低温保存鼠心的作用。方法SD雄性大鼠192只随机分为4组,即A组(单纯Celsior液保存5h)、B组(30μmol/L DE+Celsior液保存5h)、C组(30μmol/L DE+Celsior液保存8h)、D组(30μmol/L DE+Celsior液保存10h)。利用改良Heron法大鼠颈部异位心脏移植模型,供体鼠心按各组要求保存后移植于受体,观察移植心脏30s内复跳率、心率;检测心肌细胞膜Na+-K+-ATPase和肌浆网Ca2+-ATPase活性;观察心肌超微结构。结果(1)复跳率和心率:心脏移植术后30s复跳率各组均无显著性差异;B组、C组心率明显较A组、D组快,A组、D组心率差异无显著性意义。(2)两酶活性测定:B、C、D3组心肌细胞膜Na+-K+-ATPase活性随着保存时间的延长呈显著递减性降低,但仍高于A组,仅D组与A组差别无显著性意义;B组、C组肌浆网Ca2+-ATPase活性显著高于D组、A组,D组与A组差别无统计学意义。(3)心肌超微结构:各组心肌结构保护均较好,肌纤维排列整齐,肌节清,线粒体肿胀不明显;B、C、D3组线粒体嵴结构较为清楚。(4)D组和A组各指标差别均无显著性意义。结论移植鼠心在长时程冷缺血期间,DE对心肌组织ATP酶活性有较好的保护作用,此作用随着保存时间的延长而减弱。且DE处理后Celsior液冷保存鼠心10h仍与国际公认的Celsior液安全冷保存时间5h效果相当。 Objective To study whether diazoxide in the cardioplegia solution could improve the activities of sarcolemmal Na+-K+-ATPase and sarcoplasmic reticulum(SR)Ca2+-ATPase during different overtime hypothermic preservations of the rat's heart and to investigate the heart transplantation efficiency. Methods The modified Heron's technique for heterotopic cervical cardio-transplantation in rats with cuff vessel anastomosis was used. A total of 192 donor hearts were randomly stored in 4°C Celsior cardioplegia solution(group A) or Celsior solution containing diazoxide (30 μmol/L) for 5 (group B), 8 (group C) or 10 (group D) hours. The restorative rate of heartbeat and heart rate were recorded, the activities of sarcolemmal Na+-K+-ATPase and SRCa2+-ATPase were measured, and the myocardial ultrastructure was determined. Results No significant difference in the restorative rate of heartbeat was found among the 3 groups and the heart rate was better in groups B and C than that in groups A and D. Diazoxide improved the activities of sarcolemmal Na+-K+-ATPase and SRCa2+-ATPase in groups B and C. The level of sarcolemmal Na+-K+-ATPase in groups B, C, and D was significantly decreased with the preservative time prolonged, but were still higher than that in group A. No significant differences in the two ATPase were noted in group A and D. No observable histological changes were noted in any group. The protection of myocardial fibers and mitochondrial structure in group B were the best. No significant difference in any index was found in groups A and D. Conclusions Diazoxide could improve the activities of sarcolemmal Na+-K+-ATPase and SRCa2+-ATPase in the cardioplegic period and the effect was decreased gradually with the time prolonged. Celsior solution with diazoxide could safely and effectively preserve the heart for 10 hours.
出处 《中华移植杂志(电子版)》 CAS 2008年第1期30-34,共5页 Chinese Journal of Transplantation(Electronic Edition)
关键词 二氮嗪 Na+-K+-ATPase Ca2+-ATPase CELSIOR液 心肌保护 Diazoxide Na+-K+-ATPase Ca2+-ATPase Celsior Myocardial protection
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参考文献5

  • 1胡志斌,严志焜,潘晓华,贾静年,周永列.二氮嗪对移植鼠心不同时期心肌组织ATP酶活性的作用[J].浙江临床医学,2008,10(7):869-870. 被引量:1
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二级参考文献5

  • 1Lompre AM, Anger M, Levitsky D. Sarco(endo) plasmic reticulum calcium pumps in the cardiovascular system: function and gene expression. J Mol Cell Cardiol, 1994,26(9):1109- 1121.
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