氟氯西林镁阿莫西林颗粒剂的HPLC法测定

Determination of Flucloxacillin Magnesium and Amoxicillin Granules by HPLC

建立了HPLC法测定氟氯西林镁阿莫西林颗粒剂的含量。采用C18色谱柱,流动相A为0.02 mol/L磷酸二氢钾溶液(用2 mol/L氢氧化钠溶液调至pH 6.5,含1.5%溴化十六烷基三甲铵)-乙腈(60︰40),流动相B为0.02 mol/L磷酸二氢钾溶液(用2 mol/L氢氧化钠溶液调至pH 6.5,含1.5%溴化十六烷基三甲铵)-乙腈(30︰70),线性梯度洗脱;检测波长254 nm。氟氯西林和阿莫西林在250～2 500μg/ml浓度范围内线性关系良好,平均回收率为99.9%和100.4%,RSD为0.87%和0.77%。

An HPLC method was established for the determination of flucloxacillin magnesium and amoxicillin granules.A C18 column was used with mobile phase A of 0.02 mol/L potassium dihydrogen phosphate solution（adjusted to pH 6.5 with 2 mol/L sodium hydroxide solution,containing 1.5% cetyltrimethylammonium bromide）-acetonitrile（60︰40） and mobile phase B of 0.02 mol/L potassium dihydrogen phosphate solution（adjusted to pH 6.5 with 2 mol/L sodium hydroxide solution,containing 1.5% cetyltrimethylammonium bromide）-acetonitrile（30︰70） at the detection wavelength of 254 nm.The calibration curves of flucloxacillin and amoxicillin were linear in the range of 250-2 500 μg/ml.Their average recoveries were 99.9% and 100.4%,with RSDs of 0.87% and 0.77%.