4种中药体外抗口腔念珠菌的敏感性研究

In vitro anti-candidal activity of the four traditional Chinese medicines

目的测定丁香酚、柠檬醛、肉桂醛、八角茴香油的体外抗口腔念珠菌活性,为抗口腔念珠菌中药的进一步开发提供实验依据。方法收集2006年1—10月大连医科大学附属第一医院口腔科门诊38例口腔念珠菌感染患者。参照美国国家临床试验标准委员会(NCCLS)标准,用微量液基稀释法分别检测丁香酚、柠檬醛、肉桂醛和八角茴香油对38株口腔念珠菌临床分离株的最小抑菌浓度(MIC),并以氟康唑为质控药物,ATCC6258和ATCC90028为质控株控制整个实验条件。结果4种药物对白色念珠菌和克柔念珠菌的MIC分别为:丁香酚199.50～399.00mg/L和399.00mg/L;柠檬醛166.31～332.63mg/L和166.31mg/L;肉桂醛23.55～94.20mg/L和47.10mg/L;八角茴香油1218.75～2437.50mg/L和1218.75mg/L。结论丁香酚、柠檬醛、肉桂醛和八角茴香油对口腔念珠菌均有抑菌作用,其中肉桂醛的抑菌作用最强,其次为柠檬醛和丁香酚,八角茴香油最弱。

Objective To estimate in vitro anti-candidal activity of Eugenol,Citral,Cinnamaldehyde and the Essential oil of Illicium verum,in order to pick the best one which has the highest sensibility to oral Candida spp.Also,to provide a foundation for further research and development of the anti-candidal traditional Chinese medicines.Methods With the broth microdilution testing procedure proposed by NCCLS,the minimum inhibitory concentration(MIC)of the four traditional Chinese medicines were determined in 38 clinically isolated Candida strains and 2 standard strains,and Fluconazole,with ATCC 6258 and ATCC 90028 used as positive control.Results For the Candida strains the MICs of Eugenol were 199.50 ～ 399.00 mg/L for C.albicans,399.00 mg/L for C.krusei;the MICs of Citral were 166.31 ～ 332.63 mg/L for C.albicans,166.31 mg/L for C.krusei;the MICs of Cinnamaldehyde were 23.55 ～ 94.2 mg/L for C.albicans,47.10 mg/L for C.krusei;the MICs of the Essential oil of Illicium verum were 1218.75 ～ 2437.50 mg/L for C.albicans,1218.75 mg/L for C.krusei.Conclusion Eugenol,Citral,Cinnamaldehyde and the Essential oil of Illicium verum have different anti-candidal effects on 38 strains Candida spp.The effect of Cinnamaldehyde is the best,followed by Eugenol and Citral,while the Essential oil of Illicium verum is the weakest.