光学参数在FPR系统中的最佳选择

Optimization of optical parameters in FPR system

本文论述了在 FPR 系统中光学参数的最佳选择:包括激发光谱线、微光斑半径、衰减因子和荧光收集效率。

This paper describes the principe of fluoresence photobleaching recovery(FPR)te-chniques.A detailed account of optimization of optical parameters in FPR system,it is includedexcitiation spectral wavelength,radius of microspot,attenuation factor and the fluorescence collec-tion efficiency etc.