海洋微藻裂壶藻发酵液胞外多糖的制备及活性研究

Preparation of extracellular polysaccharide from fermentation liquor of marine microalgae Schizochytrium and study on the bioactivities

使用乙醇沉淀法从海洋微藻裂壶藻Schizochytrium sp.TIO1101的发酵液中提取胞外多糖,再经732阳离子交换树脂分离得到酸性多糖、弱碱性多糖和碱性多糖三种主要的多糖组分,并分别测定其还原力活性、DP-PH自由基清除活性和羟基自由基清除活性等抗氧化活性和抗菌活性.结果表明,弱碱性多糖具有最高的抗氧化活性.抑制真菌活性实验表明,三种多糖组分对苹果轮纹病菌(Physalospora piricola)、瓜果腐霉病菌(Pythi-um aphanidermatum)、葡萄灰霉病菌(Botrytis cinerea)和甜瓜枯萎病菌(Fusarium pathogens)都表现出抑菌活性,并且对葡萄灰霉病菌(Botrytis cinerea)的抑菌效果最为明显.

The ethanol precipitation was executed to extract exopolysaccharide from the fermentation solution of marine microalgae Schizochytrium sp.TIO1101.The extracted exopolysaccharide solution was then applied to 732 cationic exchange resin for further separation,the elution was isolated with into three major polysaccharide components： acidic polysaccharide,weak alkaline polysaccharides and alkaline polysaccharides.And the polysaccharides were assessed for antioxidant activity against reducing power activity,DPPH radical scavenging activity and hydroxyl radical scavenging activity assays,respectively.The results indicated that weak alkaline polysaccharides showed significant antioxidant activity.Antifungal activity experiment showed all three polysaccharide components demonstrated antifungal activity to Physalospora piricola,Pythium aphanidermatum,Botrytis cinerea and Fusarium pathogens to some extent,particularly showing high effect on Botrytis cinerea.