摘要
背景与目的:以往研究发现CD147与某些恶性肿瘤存在相关性。本研究探讨CD147在人脑胶质瘤中的表达及其与病理分级的相关性。方法:收集61例星形细胞肿瘤及18例瘤旁脑组织标本,制作组织芯片。进行免疫组织化学染色,用半定量逆转录聚合酶链反应法(RT-PCR)进行CD147mRNA检测。结果:(1)胶质瘤组织中CD147蛋白的阳性表达率为74.3%,正常脑组织中仅血管内皮细胞浆有阳性着色。(2)III、IV级星形细胞肿瘤瘤细胞胞浆CD147着色显著强于I、II级低级别星形细胞肿瘤。(3)CD147mRNA在胶质瘤组织中阳性率为83.3%,明显高于正常脑组织中的阳性率25%(P<0.05)。CD147mRNA在星形细胞肿瘤组织中的表达量为0.274±0.087,明显高于正常组织0.081±0.043,两者差异具有显著性(P<0.05)。结论:CD147的表达可能在人脑胶质瘤的发生发展中起重要作用。
BACKGROUND & OBJECTIVE:Previous studies demonstrated that CD147 expressed on the surfaces of most tumor cells and promoted invasion of tumor cells by stimulating stromal cells to produce elevated levels of several matrix metalloproteinase (MMPs). In present study, we investigate the expression of CD147 in normal brain and gliomas of different grade. METHODS: Expression of CD147 was detected in normal brain and gliomas of varying malignancy by using tissue array based on immunohistochemistry. Expression of CD147 mRNA was detected with Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). RESULT: (1) Immunolocalization revealed different distribution of CD147 in normal brain and glioma tissues: In non-neoplastic portions of the brain, CD147 immunostaining was demonstrated only in vascular endothelial cells, whereas in glioma the tumor cells were stained positively. CD147 was positive in 74.3% of glioma cells. (2) In astrocytomas, varying percentages of the tumor cells were positive, level of immunohistochemical staining of higher grade astrocytomas (grade III and IV) was higher than that of lower grade astrocytomas (grade I and II). (3) RT-PCR indicated that level of CD147 mRNA expression was higher in gliomas (0.274±0.087) than in non-neoplastic brain (0.081±0.043). Thus, the CD147 mRNA level was up-regulated significantly in gliomas compared with non-neoplastic tissue (P< 0.05). CONCLUSION: CD147 was highly expressed in gliomas and may play import role in the progression of gliomas.
出处
《中国神经肿瘤杂志》
2008年第4期228-231,共4页
Chinese Journal of Neuro-Oncology
