期刊文献+

Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families 被引量:2

Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families
原文传递
导出
摘要 Trypsin inhibitors have been found in various animals, plants and microorganisms.There were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(G.soja) and cultivated soybean(G.max) formed a multigene family.We constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART Kit.Seventeen contigs or singletons were highly homologous to soy-bean protease inhibitors.Contigs of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, respectively.Sequence analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong 14.Based on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in soybean.Phylogenetic analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor. Trypsin inhibitors have been found in various animals, plants and microorganisms.There were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(G.soja) and cultivated soybean(G.max) formed a multigene family.We constructed a cDNA library of cultivar ‘SuiNong 14' seed at the R7 growth stage using the SMART Kit.Seventeen contigs or singletons were highly homologous to soy-bean protease inhibitors.Contigs of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, respectively.Sequence analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong 14.Based on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in soybean.Phylogenetic analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor.
出处 《Science China(Life Sciences)》 SCIE CAS 2008年第8期687-692,共6页 中国科学(生命科学英文版)
基金 the "863" Project of National High Technology Research and Devel-opment Program of China (Grant No. 2006AA100104 and 2006AA10A110) Na-tional Natural Science Foundation of China (Grant No. 30490251) National Key Technologies R&D Program in the 11th Five-Year Plan (Grant No. 2006BAD13B05) Basic Research Funding of the Institute of Crop Science
关键词 SOYBEAN TRYPSIN INHIBITOR Bowman-Birk PROTEINASE INHIBITOR KUNITZ TRYPSIN INHIBITOR allelic VARIATION soybean, trypsin inhibitor, Bowman-Birk proteinase inhibitor, Kunitz trypsin inhibitor, allelic variation
  • 相关文献

参考文献2

二级参考文献4

共引文献64

同被引文献17

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部