microRNA的实验和生物信息学开发方法

The Method of Development MicroRNAs Based on Experiment and Bioinformation

mircroRNA是一类约22nt的内源小分子RNA,普遍存在于真核生物体内,它通过与靶基因互补结合来调控基因表达,从而对生物体的生长、发育等起到重要的调节作用。随着生物学技术的不断发展,mircroRNA研究的越发深入,越来越多的mircroRNA被开发出来。本文结合了mircroRNA的生理特点,从RNA、DNA和生物信息学预测这3个方面归纳总结有关开发microRNA的方法,以及由此衍生出来的相关的研究方法。通过对这些研究方法的分类总结,不但为mircroRNA以后的研究工作奠定良好的基础,而且有利于新技术的研发。

microRNAs were endogenesis small molecular RNA of 22nt in eukaryotes,and they also played an important role in regulation of development through combining with target gene to regulate gene expression.As the development of biotechnology,the research of microRNA became deeply,more and more microRNAs had been developed.This article summrized the method of developping microRNA though 3 part of RNA,DNA,and bioinformatic prediction and other research method which based on the character of physiology.With this method,more ways could be found to recognise and identify new microRNAs.