藏药山莨菪组织培养技术研究

Studies on Tissue Culture Technique in a Tibetan Medicine-Anisodus Tanguticus

以田间栽培两年生山莨菪幼芽(休眠芽)、带叶柄幼叶为外植体,通过器官培养分化芽的途径达到快速繁殖的目的。MS基本培养基附加NAA0～1.0mg·L^(-1)+6-BA2.0～3.0mg·L^(-1)+ZT0～3.0mg·L^(-1),适于丛生芽的诱导与增殖;附加2,4-D2.0mg·L^(-1)+NAA0.2mg·L^(-1)+6-BA0.2mg·L^(-1),适于愈伤组织的诱导与继代培养,诱导率高达93.2%;1/2MS培养基附加NAA1.0mg·L^(-1)+3.0%的蔗糖,适于生根诱导,生根率为100%。

To povide scientific basis for rapid-repropagation by studying the technique of tissue culture of a tibetanmedicine Anisodus tanguticus.Cluster buds,rooting and callus induction were inducted from dormancy buds and youngleaves of A.tanguticus on a MS medium supplemented with different hormones.The MS medium with 0～1.0mg·L^(-1) NAA+2.0～3.0mg·L^(-1)6-BA+0～3.0mg·L^(-1)ZT was suitable for the induction and proliferation of cluster buds. MS medium with 2.0mg·L^(-1)2,4-D+0.2mg·L^(-1)NAA+0.2mg·L^(-1)6-BA was suitable for the induction and subculture of calli.The inductivity of callus was about 93.2%.1/2MS medium with 1.0mg·L^(-1)NAA and 30g.L^(-1)sucrose was suitable for the induction of roots.The inductivity of rooting was 100%.Aseptic seeding of A.tanguticus can be quickly propagated by shoot culture.