摘要
The primary structure of atosiban(an artificially synthesized 8AA peptide) was confirmed by nanoelectrospray tandem mass spectrometry.Accurate relative molecular mass of atosiban unreduced and reduced were determined firstly by full-scan mass spectrometry,and fragmentation ions were obtained by selecting parental ions m/z 498.73(double charge) using mass spectrometry(MS/MS).The sequence and modified site of atosiban was designated by using y ion method.The method is sensitive,fast and simple,and can provide a great valuable information for confirmation of primary structure of polypeptide drugs.
The primary structure of atosiban(an artificially synthesized 8AA peptide) was confirmed by nanoelectrospray tandem mass spectrometry.Accurate relative molecular mass of atosiban unreduced and reduced were determined firstly by full-scan mass spectrometry,and fragmentation ions were obtained by selecting parental ions m/z 498.73(double charge) using mass spectrometry(MS/MS).The sequence and modified site of atosiban was designated by using y ion method.The method is sensitive,fast and simple,and can provide a great valuable information for confirmation of primary structure of polypeptide drugs.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第S1期16-18,共3页
Journal of Instrumental Analysis
