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液相色谱同位素稀释质谱对寡核苷酸含量的测定研究 被引量：4

Quantitation of the Mass of Oligonucleotides by Liquid Chromatography-Isotope Dilution Mass Spectrometry
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摘要 The mass of oligonucleotides was quantitated by liquid chromatography-isotope dilution mass spectrometry.The uncertainty was calculated.This could provide information for the further quantitative measurement and utilization of DNA. The mass of oligonucleotides was quantitated by liquid chromatography-isotope dilution mass spectrometry.The uncertainty was calculated.This could provide information for the further quantitative measurement and utilization of DNA.
作者 高运华 武利庆 王晶 张玲 刘张岚
机构地区 中国计量科学研究院
出处 《分析测试学报》 CAS CSCD 北大核心 2008年第S1期33-36,共4页 Journal of Instrumental Analysis
基金 国家基础条件平台项目(APT0718~0818-5) 质检公益性行业专项项目(AHY0709)
关键词 QUANTITATION OLIGONUCLEOTIDES liquid chromatography-isotope DILUTION mass SPECTROMETRY quantitation oligonucleotides liquid chromatography-isotope dilution mass spectrometry
分类号 R927 [医药卫生—药学]
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参考文献4

  • 1LGC.A primary method to produce standards for DNA quantitation[].National measurement system valid analytical measure-ment-chemical programme draft for public comment.2003
  • 2O‘CONNOR G,DAWSON C,WOOLFORD A,et al.Quantitation of oligonucleotides by phosphodiesterase digestion fol-lowed by isotope dilution mass spectrometry:proof of concept[].Analytical Chemistry.2002
  • 3DAWSON C,O‘CONNOR G,WOOLFORD A.Development of a primary method for DNA quantitation using liquid chro-matography-mass spectrometry(LC-MS). http://www.nla.org.za/conferences/2003/abstracts .
  • 4Sh indo Y,Kuribara H,Matsuoka T,et al.Validation of real-tim ePCR analyses for line-specific quantitation of genetically mod ifiedmaize and soybean using new reference molecu les[].Journal of AOAC International.2002

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分析测试学报
2008年 第S1期

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