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低强度超声波防治放射性骨坏死的体外研究 被引量：1

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摘要 【目的】探讨低强度超声波(LIUS)对体外培养的血管内皮细胞(VEC)和成骨细胞(OB)增殖及功能的影响。【方法】体外培养的第3代血管内皮细胞、成骨细胞以1.0×105/孔的浓度接中于6孔板内,分别接受0、1、3、6、9Gy5个剂量60Coγ射线的辐射,实验组接受低强度超声波仪的干预(功率:0.8W/cm2、频率:1.6mHz);对照组在相同的条件下低强度超声波仪的功率设为0;MTT法检测细胞的增殖情况,同时测定KDR在血管内皮细胞的表达及成骨细胞ALP的活性。【结果】电离辐射对VEC、OB的存活和增殖有抑制作用,辐射剂量为0、1、3Gy时实验组的MTT值与对照组比较有显著性差异(P<0.05);同样在0、1、3Gy辐射剂量组时,实验组的KDR在VEC中呈阳性表达;ALP活性较对照组有显著性差异(P<0.05)。【结论】LIUS对辐射后VEC和OB的增殖活性有提高作用,同时对KDR在VEC的表达及OB的ALP活性也有促进作用。
作者 陈慧芝 李祥 吴少伟 朱双喜 汪建龙
机构地区 广东药学院附属第一医院口腔科 中山大学附属第一医院黄埔院区口腔科 中山大学附属光华口腔医院种植科
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2008年第S2期35-37,共3页 Journal of Sun Yat-Sen University:Medical Sciences
关键词 低强度超声波 血管内皮细胞 成骨细胞
分类号 R818.7 [医药卫生—放射医学]
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参考文献9

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二级参考文献23

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同被引文献25

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引证文献1

中山大学学报（医学科学版）
2008年 第S2期

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