2种方法检测干细胞活率效果的比较

Comparision Between two Methods for Measuring Survival rate of stem cell

目的:比较碘化丙锭(PI)拒染试验和台盼蓝拒染试验对干细胞活率检测的效果。方法:选择18例干细胞动员后的采集物,在采集后的第1、3、5、7、9、11天,同时进行PI拒染试验和台盼蓝拒染试验。结果:PI拒染率与台盼蓝拒染率结果差异均无统计学意义,相关性检验显示2者呈显著正相关(r=0.996);精密度检验显示,PI拒染试验优于台盼蓝拒染试验(平均CV1.46%vs5.10%)。结论:PI拒染试验在干细胞活率检测中优于台盼蓝拒染试验。

Objective:To compare the efficiencies of Propidium Iodide(PI) excluding test and trypan blue excluding test in measuring survival rate of stem cell.Method:18 acquisition samples were measured by PI excluding test and trypan blue excluding test on the first,third,fifth,seventh,ninth,eleventh day.Result:The results of two measure methods had no significant difference.There was a positive significant correlation between the results of two methods.The precision of PI excluding test was better than that of trypan blue excluding test.Conclusion:These findings suggested that efficiency of PI excluding test was more excellent than that of trypan blue excluding test in measuring survival rate of stem cell.