摘要
目的建立大鼠血浆和尿液中赛特铂含量测定的无火焰石墨炉原子吸收分光光度法。方法采用岛津AA-670原子吸收分光光度仪及GFA-4A石墨炉,上海电光KY-1铂空心阴极灯,检测波长265.9 nm,测定大鼠血浆和尿液中的赛特铂含量。血尿样品用Triton X-100稀释,进样50μL。结果在浓度范围0.25 mg.L-1~10.0 mg.L-1内,样品峰面积与浓度呈良好线性关系,相关系数r2分别为血浆0.9952,尿液0.9878;加样回收率为血浆89%~112%,尿液82%~100%。结论方法简便可靠,适于生物样本中的赛特铂含量测定和动物药动学研究。
OBJECTIVE To develop a rapid,accurate and sensitive method for the measurements of satraplatin in biological fluids of rats.METHODS Satraplatin in plasma and urine was analyzed by flameless atomic absorption spectrometry on an instrument Shimadzu AA-670 atomic absorption spectrophotometer equipped with a Shimadzu GFA-4A graphite furnace,and an ASC-60 autosampler.The detection wavelength was set at 265.9 nm.Plasma and urine samples were diluted with Triton X-100 before assays,fifty microliters of solution after preparation was injected to the system. RESULTS The linear ranges of satraplatin in plasma or urine were 0.25mg·L^(-1)~10.0mg·L^(-1).The correlation coefficient r^(2) was 0.9952 and 0.9878,and the regression equation were A=(0.2118)C-0.0548 and A=0.1556C-0.0042 for plasma and urine,respectively.The average recoveries were 89%~112% in plasma and 82%~100% in urine,respectively.The RSD values of interday and intra-day assays were lower than 8.9% and 6.4% respectably.CONCLUSION This method is suitable for the preclinical pharmacokinetic studies of satraplatin in rats.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第S2期731-733,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
赛特铂
大鼠
血浆
尿
原子吸收分光光度法
铂抗癌药
satraplatin
rats
plasma
urine
atomic absorption spectrometry
oral platinum anticancer drug