双波长分光光度法测定复方地氯霜中氯霉素的含量

Determination of chloramphenicol in Dexamethasone and Chloramphenicol Compound Cream by Dual-Wavelength Spectrophotometry

目的建立复方地氯霜中氯霉素的含量测定方法。方法采用双波长分光光度法测定氯霉素的含量。选择测定波长为268nm,参比波长为248nm。结果氯霉素的平均回收率为104.3%,RSD为2.93%(n=6)。结论该方法简便、准确,适用于该制剂的质量控制。

OBJECTIVE To establish a method for the determination of chloramphenicol in dexamethasone and chloramphenicol compound cream.METHODS A dual-wavelength spectrophotometry was used.The selected determining wavelength was 268nm and the reference wavelength was 248nm. RESULTS The average recovery of chloramphenicol was 104.3%,RSD=2.93%(n=6).CONCLUSION This method is simple,accurate and suitable for assay of the preparation.