氨溴索对罗红霉素大鼠体内药动学的影响

Effect of aebroxol on The Pharmacokientice of Roxithromycin in Rats

目的:研究氨溴索(Amb)对罗红霉素(RM)大鼠体内药代动力学的影响,探讨药物间的相互作用及联合用药的合理性。方法:72只SD大鼠随机分为4组,给药前禁食12h。对照组的大鼠单独予RM;实验组Ⅰ、Ⅱ、Ⅲ分别预先给药2.7mg.kg-1、5.4mg.kg-1、10.8mg.kg-1剂量的Amb,tid×6d,于第7d给药Amb+RM。给药后经眼眶静脉丛采集血样,样品处理后采用反相HPLC-UV检测法测定不同时间RM的血药浓度,采用DAS2.1.1统计软件求算该药单用及联合用药后的药动学参数。结果:采用随机区组的方差分析进行统计学处理,实验组与对照组各组间RM的主要药动学参数Tmax、T1/2z、Cmax、Vz、CLz、AUC0～t、AUC0～∞差异均无统计学意义(p>0.05)。结论:Amb与RM联合使用时,Amb对RM大鼠体内药代动力学过程无明显影响,二者联合使用安全,本结果为临床安全有效地使用Amb和RM提供了一定的实验依据。

OBJECTIVE：In order to evaluate the interactions and rationality of drug combination in clinic between Amb and RM,the influences of Amb on the pharmacokinetic parameters of RM in rats were investigated in this research. METHOD：72 SD rats were randomly divided into 4 groups.The RM was given to the control group while Amb which were at doses of 2.7mg· kg-1,5.4 mg·kg-1,10.8 mg·kg-1 were given to experimental group Ⅰ,Ⅱ and Ⅲfor 6 days,and given together with RM at the seventh day respectively.Blood samples were collected by orbital plexus.The RM concentrations in the plasma were determinated by HPLC-UV,and the pharmacokinetic parameters of RM were calculated by DAS 2.1.1. RESULTS：The results showed that there were no significant differences on the main pharmacokinetic parameters of RM such as Tmax,T1/2z,Cmax,Vz,CLz,AUC0～t and AUC0～∞ between the experimental groups and the control group（p〉0.05）. CONCLUSION：The Amb did not have significant influences on the pharmacokinetic of RM in rats.It is safe to use the combination of the two drugs and there is no need to adjust the dosage of RM.This study provided a certain experimental evidence for clinical use of RM when co-administrated with Amb.