男性不育症患者精液ADAM基因表达的临床意义

Research on the ADAM gene expression in patient with infertility

目的探讨ADAM(去整合素和金属蛋白酶区家族)基因与不明原因不育症之间的关系。方法应用RT-PCR方法检测30例正常生育男性和30例不明原因不育症患者精液中ADAM1、ADAM2、ADAM3、ADAM32基因的 mRNA表达。结果正常组30例中4种基因 mRNA均有表达,不育组30例中有1例ADAM1、2、32呈阴性表达,1例ADAM2、32呈阴性表达,ADAM1和ADAM3呈阴性表达各1例,26例4种基因均呈阳性表达。结论ADAM1、2、3、32基因缺失或不表达可能是导致不育的候选基因之一。

Objective To investigate the relationship between the ADAM gene expression and unknown reason infertile patients. Methods With RT-PCR mehtod, we checked from normal group semen 30 cases and infertile group semen 30 cases in order to know the ADAM1,2,3,32 mRNA expression. Results there are the ADAM1,2,3,32 gene expression in all 30 cases of normal group whereas in 30 cases infertile patients there exists 1 case ADAM1,2,32 lacking expression, 1 case ADAM2,32 lacking expression, 1 case ADAM1 and 1 case ADAM3 lacking expression. Conclusions The lacking of ADAM1,2,3,32 may be one of the most reasons which cause the infertility.