RP-HPLC法测定高原地区人血浆中利多卡因的血药浓度

Determination of lidocaine in human plasma of high-altitude area by RP-HPLC

目的:建立用RP-HPLC法测定人血浆中利多卡因的分析方法。方法:采用RP-HPLC法将利多卡因同血浆杂质分离,以利多卡因和内标峰面积的比值对其浓度进行线性回归,计算血药浓度。色谱柱为LichrospherC18(4.6×250mm,5μm),流动相为甲醇-水-冰醋酸-三乙胺(62:38:0.3:0.6),紫外检测波长为。结果:在0.1～6mg·L-1范围内利多卡因峰面积与内标峰面积之比与浓度线性关系良好,最低检测浓度为0.03mg·L-1,回收率利多卡因为91.40%～95.5%,日内RSD为3.8%～9.4%、日间RSD为4.2%～10.3%。结论:样品处理简便快速,测定方法准确可靠,适合于高原地区利多卡因临床治疗药物监测及药代动力学研究。

To develop a simple,sensitive and rapid method for the determination of lidocaine in human plasma. The concentration of lidocaine and tetracaine was determined by RP-HPLC method,the analysis involved a 250 mm ×4.6mm column packed with C18 (5μm),the mobile phase consisted of methyl alcohol-water-glacial acetic acid-triethylamine(62:38:0.3:0.6),and ultraviolet wavelength for detection is 220 nm.Result:A good linearity was obtained in the range of 0.1 mg.L^(-1)～6 mg·L^(-1),the recovery of lidocaine and tetracaine were between 91.4%～ 95.5%,the intra-day RSD is 3.8%～9.4% and the inter-day is 4.2%～10.3%,and the minimal detectable concentra- tion in plasma was 0.03 mg·L^(-1).Conclusion:The results demonstrated that the method was sensitive,accurate and simple for the determination of lidocaine in human plasma,and it is suitable for the pharmacokinetics study and clini- cal therapeutic monitoring of lidocaine in high-altitude area.