山豆根中苦参碱不同提取方法的对比研究

Comparative Studies on Different Methods for The Extraction of Matrine in Root of Subprostrate Sophora

目的:本实验主要考察了传统方法与超临界Co_2萃取方法从山豆根中提取生物碱的工艺的优劣,从而确定从山豆根中提取苦参碱的最佳方法。方法:分别采用渗漉法、温浸法两种传统方法和Co_2超临界萃取法.提取山豆根中苦参碱,运用高效液相色谱法,以苦参碱为标准品,测定各提取物中苦参碱的含量,从而确定山豆根中提取苦参碱的最佳方法。结果:从超临界萃取万法萃取的苦参碱含量比渗漉法高出0.072%,比温浸法高出0.105%可看出,超临界萃取方法萃取的苦参碱的含量要高于传统方法。结论:超临界萃取方法可为从山豆根中提取苦参碱的较理想的方法。

Aim: To evaluate the process for the extracting of matrine from root of subprostrate sopf}ora by traditional and supercritical C02 extraction methods and to find the optical process. Methods: Two traditional methods,percolation and warm dipping, and supercritical C02 extracting was used for the extraction of matrine from root of subprostrate sophora. HPLC was used to determine the content of matrine extracted by different methods with matrine as reference. Results:The content of matrine extracted by supercritical C02 was 0.072% times higher than that by percolation, and 0.105% times higher than that by warm dipping. Concluslon:Supercritical C02 method was ideal for the extracting of matrine from root of subprostrate sophora.