蒙药胃舒安胶囊中栀子苷的含量测定

Determination of Geniposide in Weishu' an Capsules by HPLC

目的:制定蒙药胃舒安胶囊的含量测定质量标准。方法:用高效液相色谱法对方中的栀子主要成分栀子苷进行定量分析。采用 VP-ODS(150L*4.6)柱,乙腈-0.1%磷酸水溶液(15:85V/V)为流动相,检测波长为238nm,流速为1.0ml.min^(-1)。结果:栀子苷进样量在0.138-0.690μg范围内与峰面积线性关系良好(r=0.99987)。平均加样回收率为102.98%,RSD=0.60%。结论:本试验方法即先进又简便、结果准确可靠为该药制定含量测定的质量标准提供可靠依据。

An HPLC method for determination of geniposide in Weishu'an Capsules was established. Methods:The analysis was performed with a VP-ODS(150L*4.6)conlumn and a mobile phase consisting of CH3CN-0.1%H3PO4(15:85V/V)eluted at 1.0ml.min^(-1).The detection wavelength was at 238nm.Results:The calibration curves were linear for geniposide in 0.138-0.69ug(r=0.9998).The recovery rate was 102.98%(RSD= 0.60%).Conclusion:The method was simple with a satisfactory separating effect.