摘要
Proteomic approaches using two-dimensional gel electrophoresis （2-DE） were adopted to identify proteins from cucumber leaves differentially expressed in F2 generation of cucumber. Nearly 500 proteins were reproducibly detected, including 39 specific proteins expressed in F2 sensitive pool samples and 22 specific proteins expressed in resistance pool samples, 35 proteins were two-fold upper limit and 46 proteins were two-fold lower limit. Furthermore, analysis with MALDI-TOF-MASS showed that one important protein of disease resistance gene （SSP3110） identified in F2 generation of cucumber associated with defensive reaction. Three proteins （SSP5412, SSP5513, SSP5710） related to respiration or photosynthesis were identified in F2 resistant pool samples, and 14-3-3 brain protein homolog （SSP1411） was identified in F2 sensitive pool samples. All those proteins were probably parts of network of resistance or development-related proteins. This study gives new insights into cucumber powdery mildew resistant response in cucumber leaves and demonstrates the power of the proteomic approach in plant biology studies. MS analysis of other protein spots is underway.
Proteomic approaches using two-dimensional gel electrophoresis (2-DE) were adopted to identify proteins from cucumber leaves differentially expressed in F_2 generation of cucumber. Nearly 500 proteins were reproducibly detected, including 39 specific proteins expressed in F_2 sensitive pool samples and 22 specific proteins expressed in resistance pool samples, 35 proteins were two-fold upper limit and 46 proteins were two-fold lower limit. Furthermore, analysis with MALDI-TOF-MASS showed that one important protein of disease resistance gene (SSP3110) identified in F_2 generation of cucumber associated with defensive reaction. Three proteins (SSP5412, SSP5513, SSP5710) related to respiration or photosynthesis were identified in F_2 resistant pool samples, and 14-3-3 brain protein homolog (SSP1411) was identified in F_2 sensitive pool samples. All those proteins were probably parts of network of resistance or development-related proteins. This study gives new insights into cucumber powdery mildew resistant response in cucumber leaves and demonstrates the power of the proteomic approach in plant biology studies. MS analysis of other protein spots is underway.
基金
Item supported by the national natural sci-ence foundation of China (No.30370917 and No.30471057)
the key project of science and technology commission of Shanghai(No.043919364)
