螺旋藻Spirulina在鄂尔多斯碱湖的越冬机制

MECHANISM FOR Spirulina LIVE THROUGH WINTER IN ALKALINE LAKE OF ERDOS

为了了解螺旋藻在鄂尔多斯沙区碱湖的越冬机制,选择4种分布在鄂尔多斯碱湖螺旋藻和国外引进的11个种类和族群的螺旋藻,使用比色法、冰冻复生法和实地调查法测试了螺旋藻的低温适应性、冰点和复生的机制。结果表明:本土钝顶螺旋藻低温性明显优于引进钝顶螺旋藻,但是当温度低于4℃本土钝顶螺旋藻也处于不断的分解中;本土螺旋藻的冰点在-8℃左右,其抗冻能力优于引进种。被冰冻过的螺旋藻其复生与藻种本身的抗冻能力以及复生的条件相关,引进的螺旋藻不具备在鄂尔多斯碱湖越冬的能力,所以也无生态入侵的危险。实验发现冰冻过的螺旋藻溶化后,室温条件下24h之内都被分解成无细胞状态的物质,复生是从这些物质开始的,这一发现可为螺旋藻的基因工程和遗传育种开创新路径。

4 species native Spirulina,11 species and strains introduced Spirulina were selected to study on Spirulina Living through winter in Alkaline lake of Erdos.The colorimetric method and the reviving from freezing and investigating lakes of Spirulina were used to test Spirulina＇s low temperature adaptability and freezing point and reviving mechanism.The results show that adaptability to low temperature of native S.platensis was better than introduced S.platensis,but native S.platensis decompsed continuously as temperature below 4℃.The freezing point of native Spirulina was-8℃ or so and their ability of anti-freezing is better than the introduced.Reviving from freezing is related with Spirulina ability of anti-freezing and the condition of their reviving.Introduced Spirulina could not live through winter and there is not a danger of ecological inbreak in Alkaline lake of Erdos.The experiment discovered that Spirulina after freezing was decompsed into no cells substance in 24 hours at room temperature,and the reviving came form the no cells substance.This discovery could give a new way for gene engineering and heredity breeding of Spirulina.