期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Construction of Yeast Vectors with Resistance to Geneticin 被引量：3

Construction of Yeast Vectors with Resistance to Geneticin
原文传递
导出
摘要 Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglycoside 3' phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains. Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglycoside 3' phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains.
作者 林会兰 张广 周全 陈国强
出处 《Tsinghua Science and Technology》 SCIE EI CAS 2002年第4期384-386,392,共4页 清华大学学报（自然科学版（英文版）
关键词 geneticin (G418) shuttle vector green fluorescent protein (GFP) Escherichia coli (E. coli) Saccharomyces cerevisiae geneticin (G418) shuttle vector green fluorescent protein (GFP) Escherichia coli (E. coli) Saccharomyces cerevisiae
分类号 Q932 [生物学—微生物学]
  • 相关文献

参考文献16

  • 1C. Hadfield,B. E. Jordan,R. C. Mount,G. H. J. Pretorius,E. Burak.G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae[J]. Current Genetics . 1990 (4)
  • 2Chen X J.Low-and high-copy number shuttle vectors for replication in the budding yeast Kluyveromyces lactis. Gene . 1996
  • 3Mumberg D,Muller R,Funk M.Yeast vectors for the controlled expression of heterologous proteins in different backgrounds. Gene . 1995
  • 4Sikorski R S,Hieter P.A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics . 1989
  • 5Malagon F,Aguilera A.Construction and genetic analysis of S.cerevisiae deletants of six novel ORFs from chromosome II. Yeast . 1999
  • 6Vandenberg M A,Steensma H Y.Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae. Yeast . 1997
  • 7Burke J F,Mogg A E.Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G418 and paromomycin. Nucleic Acids Research . 1985
  • 8Scorer C A,Clare J J,McCombie W R.Rapid selection using G418 of high copy number transformants of Pichia pastoris for high level foreign gene expression. Journal of Biotechnology . 1994
  • 9Adams A,Gottschling D E,Kaiser C A,Stearns T.Methods in Yeast Genetics. . 1997
  • 10Wach A.PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S.cerevisiae. Yeast . 1996

同被引文献40

  • 1陈平华,陈如凯,高三基.甘蔗愈伤组织G418最低抑制浓度的测定[J].江西农业大学学报,2005,27(1):63-67. 被引量：8
  • 2郑荣,朱宝泉.透明颤菌来源的血红蛋白基因的研究及应用进展[J].中国医药工业杂志,1994,25(10):472-477. 被引量：3
  • 3林美娟,薛志平,陈平华,高三基,陈如凯.抗生素G418在甘蔗组织培养中的最佳筛选浓度[J].福建农林大学学报（自然科学版）,2006,35(1):6-10. 被引量：10
  • 4Tian S P, Fan Q, Xu Y, et al. Biocontrol efficacy of antagonist yeasts to grey mold and blue mold on apples and pears in controlled atmospheres[J]. Plant Disease. 2002, 86(8):848-853.
  • 5Tian S P, Qin G Z, Xu Y. Survival of antagonistic yeasts under field condition and their biocontrol ability to postharvest diseases in sweet cherry[I]. Postharvest Biology and Technology, 2004, 33(3): 327-331.
  • 6E1-Ghaouth A, Smilanick J L, Wisniewski M. et al. Improved control of apple and citrus fruit decay with a combination of Candida saitoana and 2-Deoxy-D-glucos[J]. Plant Disease, 2000, 8 (3):249-253.
  • 7Teresa S, Ndez J F, Vicente-Soler J, et al. Accumulation of trehalose by overexpression of tpsl, coding for trehalose-6-phosphate synthase, causes increased resistance to multiple stresses in the fission yeast Schizosaccharomyees pombe[J]. Applied and environmentalmicrobiology,, 1999, 65(5):2020 - 2024.
  • 8Limon M C, Pintor-Toro J A, Benitez T. Increased antifungal activity of Tnchoderma hamianum transformants that overexpress a 33-kDa chitinase[l]. PhytopathoIogy 1999, 89(3):254-261.
  • 9Kwon H B, Yeo E T, Hahn S E, et al. Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii. FEMS Yeast Research. 2003, 3 (4): 433-440.
  • 10Masih E I, Paul B. Secretion of beta-1,3-glucanases by the yeast Pichia membranifaciens and its possible role in the biocontrol of Botrytis cinerea causing grey mold disease of the grapevine[J]. Current Microbiology. 2002, 44 (6):391-395.

引证文献3

二级引证文献3

Tsinghua Science and Technology
2002年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部