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Heat Denaturation of Protein Structures and Chlorophyll States in PSII Membranes

Heat Denaturation of Protein Structures and Chlorophyll States in PSII Membranes
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摘要 Heat denaturation is an important technique in the study of the structure and function of photosynthetic proteins. Heat denaturation of photosystem II (PSII) membrane was studied using circular dichroism (CD) spectroscopy, differential scanning calorimetry (DSC) and oxygen electrode. Complete loss of oxygen evolving activity of the PSII membrane was observed at temperatures below 45℃ . The decrease of excitonic interaction between chlorophyll molecules occurred more rapidly than the change of the protein secondary structure of the PSII membrane at temperatures above 45℃ . The results indicate that the protein secondary structure of the membrane proteins in PSII membranes is more stable than the excitonic interaction between chlorophyll molecules during heat denaturation. Heat denaturation is an important technique in the study of the structure and function of photosynthetic proteins. Heat denaturation of photosystem II (PSII) membrane was studied using circular dichroism (CD) spectroscopy, differential scanning calorimetry (DSC) and oxygen electrode. Complete loss of oxygen evolving activity of the PSII membrane was observed at temperatures below 45℃ . The decrease of excitonic interaction between chlorophyll molecules occurred more rapidly than the change of the protein secondary structure of the PSII membrane at temperatures above 45℃ . The results indicate that the protein secondary structure of the membrane proteins in PSII membranes is more stable than the excitonic interaction between chlorophyll molecules during heat denaturation.
出处 《Tsinghua Science and Technology》 SCIE EI CAS 2002年第4期407-410,共4页 清华大学学报(自然科学版(英文版)
基金 Supported by the State Key Basic Research and Development Plan (No.G19980 10 10 0 ) the National Natural Science Foundation of China(No.3 9890 3 90 )
关键词 photosystem II (PSII) oxygen evolving activity circular dichroism (CD) differential scanning calorimetry (DSC) excitonic interaction heat denaturation photosystem II (PSII) oxygen evolving activity circular dichroism (CD) differential scanning calorimetry (DSC) excitonic interaction heat denaturation
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参考文献13

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