期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Effects of CIITA antisense RNA on the expression of HLA class Ⅱ molecules 被引量：2

Effects of CIITA antisense RNA on the expression of HLA class Ⅱ molecules
原文传递
导出
摘要 To study the effect of the major histocompatibility complex class Ⅱ (MHC Ⅱ ) transactivator (CIITA) antisense RNA on the expression of the human leukemia (HLA) class Ⅱ molecules, 5’ end cDNA sequence of CIITA gene was cloned, and antisense RNA expression vector pcDNA-Ⅱ was constructed. HeLa cells transfected with pcDNA-Ⅱ and pcDNA3 were induced by IFN-γ for 3 d. The expression of HLA class Ⅱ molecules on HeLa/pcDNA-Ⅱ cells was significantly decreased, while it has no effect on the expression of HLA class Ⅰ molecules. This result suggests that the CIITA antisense RNA can inhibit the expression of HLA class Ⅱ molecules in HeLa cells. It also implies a promising approach to generate immune tolerance in qraft transplantation. To study the effect of the major histocompatibility complex class Ⅱ (MHC Ⅱ ) transactivator (CIITA) antisense RNA on the expression of the human leukemia (HLA) class Ⅱ molecules, 5' end cDNA sequence of CIITA gene was cloned, and antisense RNA expression vector pcDNA-Ⅱ was constructed. HeLa cells transfected with pcDNA-Ⅱ and pcDNA3 were induced by IFN-γ for 3 d. The expression of HLA class Ⅱ molecules on HeLa/pcDNA-Ⅱ cells was significantly decreased, while it has no effect on the expression of HLA class Ⅰ molecules. This result suggests that the CIITA antisense RNA can inhibit the expression of HLA class Ⅱ molecules in HeLa cells. It also implies a promising approach to generate immune tolerance in qraft transplantation.
作者 ZHOU Caihong, LU Daru, ZHU Qiquan, QIU Xinfang & XUE JinglunInstitute of Genetics, Fudan University, Shanghai 200433, China
出处 《Chinese Science Bulletin》 SCIE EI CAS 2000年第22期2068-2071,共4页
关键词 CIITA ANTISENSE RNA HLA CLASS Ⅱ molecules. CIITA, antisense RNA, HLA class Ⅱ molecules.
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献8

  • 1Chin KC,Li G,Ting JP.Activation and transdominant suppression of MHC clssⅡand HLA-DMB promoters by a series of C-terminal classⅡtransactivator deletion mutants. J Immunol . 1997
  • 2Benichou,G.Direct and inditect antigen recognition: the pathways to allograft immune rejection. Frontiers in Bioscience . 1999
  • 3Fairchild,P.J.Presentation of antigenic peptides by products of the major histocompatibility complex, J. Peptide Science . 1998
  • 4Boss,J. M.Regulation of transcription of MHC class Ⅱ genes. Current Opinion in Immunology . 1997
  • 5Muhlethaler-Mottet A,Steimle V.Expression of MHC class Ⅱ mole-cules in difierent cellular and functional compartments is controlled by differential usage of multiple promoters of transactivator CⅡ TA. EMBO Journal . 1997
  • 6Steimle, V,Otten, L. A,Zufferey, M.Complementation cloning of an MHC class Ⅱ transactivator mutated in hereditary MHC class Ⅱ deficiency (or bare Iymphocyte syndrome). Cell . 1993
  • 7Chang, C. H,Flavell, R. A.Class Ⅱ transactivator regulates the expression of multiple genes involved in antigen presentation. The Journal of Experimental Medicine . 1995
  • 8Brodsky, F. M,Lem, L,Bresnaham, P.A. et al.Antigen processing and presentation. Tissue Antigens .

同被引文献20

  • 1Sisk T J, Roys S, Chang CH. Self-Association of C TA and Its Transactivation Potential [J]. Molecular and Cellular Biology,2001,21 (15):4919-4928.
  • 2Casoli C, De Lerma Barbaro A, Pilotti E, et al. The MHC class II transcriptional activator (CIITA) inhibits HTLV-2 viral replication by blocking the function of the viral transactivator Tax-2[J]. Blood,2004, 103(3):995-1001.
  • 3Raval A, Weissman JD, Howcroft TK, et al. The GTP-binding domain of class II transactivator regulates its nuclear export [J]. J Immunol, 2003,170(2):922-930.
  • 4Hake SB, Mastemak K, Kammerbauer C, et al. C II TA Leucine-Rich Repeats Control Nuclear Localization, In Vivo Recruitment to the Major Histocompatibility Complex (MHC) Class II Enhanceosome, and MHC Class II Gene Transactivation [J]. Molecular and Cellular Biology,2000,20(20):7716-7725.
  • 5Cressman DE, William J. O~ Connor, Susanna F. Greer, et al. Mechanisms of nuclear import and export that control the subcellular localization of class II transactivator [J]. J Immunol,2001,167(7):3626 -3634.
  • 6Li GX, Harton JA, Zhu XS, et al. Downregulation ofC II TA Function by Protein Kinase A (PKA)-Mediated Phosphorylation: Mechanism of Prostaglandin E, Cyclic AMP, and PKA Inhibition of Class II Major Histocompatibility Complex Expression in Monocytic Lines [J]. Mol- ecular and Cellular Biology,2001,21(14):4626-4635.
  • 7Zhu XS, Linhoff MW, Li GX, et al. Transcriptional Scaffold: CIITA Interacts with NF-Y, RFX, and CREB To Cause Stereospecific Regul- ation of the Class II Major Histocompatibility Complex Promoter [J]. Molecular and Cellular Biology,2000,20(16):6051-6061.
  • 8Harton JA, Ting PY. Class II Transactivator: Mastering the Art of Major Histocompatibility Complex Expression [J]. Molecular and Cellular Biology.2000.20( 17):6185-6194.
  • 9Holling TM, Stoep N, Quinten E, et al. Activated Human T Cells Accomplish MHC Class II Expression Through T Cell-Specific Occupation of Class II Transactivator Promoter III [J]. The Journal of Immunology,2002,168:763-770.
  • 10Athena WW, Ghosh N, Karen P. McKinnon, et al. Regulation and Specificity of MHC2TA Promoter Usage in Human Primary T Lymphocytes and Cell Line [J]. The Journal of Immunology,2002,169:3112-3119.

引证文献2

Chinese Science Bulletin
2000年 第22期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部