摘要
Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each individual. The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty--nine of 106 loci detected were polymorphic, amounting to 36. 8 %. Mean genetic distance was 0. 094 1 0. 020 6; 68 markers (63. 2 % of the total) showed stable homogeneity in all of the 32 individuals.
Genetic diversity of 32 individuals of P. chinensis in the Chinese coastal waters of the Huanghai and Bobal Seas was detected by RAPD technique. Twenty decamer primers of OPI were used for DNA amplification for each individual. The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty--nine of 106 loci detected were polymorphic, amounting to 36. 8 %. Mean genetic distance was 0. 094 1 0. 020 6; 68 markers (63. 2 % of the total) showed stable homogeneity in all of the 32 individuals.
基金
the National Natural Science Foundation of China finder the Project! No. 39620260.
