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Molecular recognition: monomer of the yeast transcriptional activator GCN4 recognizes its dimer DNA binding target sites specifically 被引量：1

Molecular recognition: monomer of the yeast transcriptional activator GCN4 recognizes its dimer DNA binding target sites specifically
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摘要 It is widely believed that dimerization is a requirement for the yeast transcriptional activator GCN4 to recognize its specific DNA target sites. We used the basic region (226-252) of the yeast transcriptional activator GCN4, as both a monomeric peptide and a disulfide-linked dimer to investigate the interaction of the peptides with the DNA target sites AP-1 and CRE. CD and ITC experiments indicate that although the monomeric peptide GCN4-M has a weaker affinity with the DNA relative to the disulfide-linked dimer peptide GCN4-D, it recognizes AP-1 and CRE target sites specifically. It is widely believed that dimerization is a requirement for the yeast transcriptional activator GCN4 to recognize its specific DNA target sites. We used the basic region (226-252) of the yeast transcriptional activator GCN4, as both a monomeric peptide and a disulfide-linked dimer to investigate the interaction of the peptides with the DNA target sites AP-1 and CRE. CD and ITC experiments indicate that although the monomeric peptide GCN4-M has a weaker affinity with the DNA relative to the disulfide-linked dimer peptide GCN4-D, it recognizes AP-1 and CRE target sites specifically.
作者 曹炜 刘亮 来鲁华 唐有祺
机构地区 Institute of Physical Chemistry
出处 《Science China Chemistry》 SCIE EI CAS 2000年第5期466-476,共11页 中国科学（化学英文版）
关键词 molecular recognition conformational change thermodynamic parameter ITC CD molecular recognition conformational change thermodynanlic parameter ITC CD.
分类号 O631 [理学—高分子化学]
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参考文献10

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同被引文献8

引证文献1

  • 1LI ZhiLiang1,2, WU ShiRong1,2, CHEN ZeCong1,2, YE Nancy1,2, YANG ShengXi1,2, LIAO ChunYang1,2, ZHANG MengJun1,2,3, YANG Li1,2, MEI Hu1,2,4, YANG Yan1,2, ZHAO Na1,2, ZHOU Yuan1,2, ZHOU Ping1,2, XIONG Qing1,2, XU Hong1,2, LIU ShuShen1,2, LING ZiHua1,2, CHEN Gang1,2,4 & LI GenRong1,2 1 College of Chemistry and Chemical Engineering/Key Laboratory for Chemobiomedical Science and Engineering under Chongqing Municipality, College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education, Chongqing University, Chongqing 400044, China,2 State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at Hunan University, Changsha 410012, China,3 Department of Medical Analysis/PLA Center of Bioinformatics Immunology, Surgeon Third University, Chongqing 400031, China,4 Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore.Structural parameterization and functional prediction of antigenic polypeptome sequences with biological activity through quantitative sequence-activity models (QSAM) by molecular electronegativity edge-distance vector (VMED)[J].Science China(Life Sciences),2007,50(5):706-716. 被引量：1

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Science China Chemistry
2000年 第5期

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