中国棉铃虫单粒包埋型核多角体病毒gp^(37)基因的序列分析

Sequence Analysis of the gp^(37)Gene of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus

昆虫杆状病毒的 gp37基因与昆虫痘病毒纺锤体蛋白 (fusolin)基因具有较高的同源性。本研究通过末端测序法和引物步移法双向测定了中国棉铃虫单粒包埋型核多角体病毒 (HaSNPV)gp37基因的核苷酸全序列。HaSNPV的 gp37基因开放阅读框为 84 0个核苷酸 ,共编码 2 79个氨基酸 ,预计蛋白质分子量为 31kDa。在 gp37基因起始密码子上游有一典型的杆状病毒晚期转录启动子结构GTAAG和两个TATAboxes。HaSNPVGP37前 18个氨基酸有很强的疏水性 ,可能是分泌信号肽序列。 16种GP37/Fusolin的氨基酸序列同源性比较分析表明 ,HaSNPVGP37与其它gp37基因的同源性较高 ( 4 0 60 % ) ,与痘病毒的Fusolin的同源性达 30 % 4 0 %左右。这些蛋白有 5个高度保守区 ,预计这 5个保守区为GP37/Fusolin的功能区。

The gp37 gene in baculovirus and the fusolin gene are two homologous genes. In this paper the gp37 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus have been identified and sequenced by using the terminal and primer walking sequencing methods. The open reading frame of the HaSNPV gp37 gene is 840 nucleotides long, encoding a putative protein of 279 amino acid with an Mr of about 31 kD. The 5' nonencoding region upstream of a putative translational start site contains one late consensus motif (GTAAG) and two TATA boxes. The HaSNPV gp37 gene has 40 56% amino acid identity to other baculovirus gp37 genes and 30 40% to entomopoxvirus fusolin genes. The 18 N terminal amino acid (aa) residues were hydrophobic and could act as a signal peptide. In amino acid sequence comparisons between GP37 and Fusolin, 5 conserved regions of very high amino acid conservation were identified. These conserved regions may act as the function regions of the protein.