球形芽孢杆菌和苏云金芽孢杆菌以色列亚种杀蚊毒素间的协同作用

Synergism Reaction Between Mosquital-larvicidal Genes from Bacillus sphaericus and B.thuringiensis subsp. israelensis

本研究测定了分别表达苏云金芽孢杆菌Cry4Aa、Cry4Ba、Cry11Aa、Cyt1Aa和球形芽孢杆菌二元毒素Bin的转化菌株Bt B60 1、Bt B611、Bt B640、Bt U 30和Bt CW 3全发酵培养物两两或两两以上不同组合对抗性库蚊的毒力 ,分析了杀蚊毒素间的协同作用。结果表明 ,Bin和Cry4Aa、Bin和Cry 4Ba间有明显的协同作用 ,此外 ,Cry4Aa和Cry4Ba、Cry4Aa和Cry11Aa。

Mixtures of the final whole cultures of recombinant strains Bt B601, Bt B611, Bt B640, Bt U 30(expressing Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa protein of Bacillus thurigiensis subsp. israelensis respectively) and Bt CW 3(expressing the Binary toxin of Bacillus sphaericus ) were used in bioassays against resistant Cpq larvae to value the synergism among the mosquito larvicidal toxins. Synergy was clearly evidenced. In particular, there were synergistic interactions between Binary toxin and Cry4Aa, between binary toxin and Cry4Ba, Cry4Aa and Cry4Ba, Cry4Aa and Cry11Aa, and between Cyt1Aa and Cry4Aa.