摘要
铜绿微囊藻 (Microcystisaeruginosa)CCAP1450 / 4在室内人工培养。结果表明在较大培养液表面、连续适当光照 ( 2 0 μE- 2 S- 1)及适宜的温度 ( 2 5℃ )有利于该藻菌的生产。通过超声波击碎细胞 ,离心过滤 ,C18净化柱分离 ,得到铜绿微囊藻毒素。应用高效液相色谱分离主峰得到纯化铜绿微囊藻毒素LR ,该毒素LR在紫外光谱分析中 ,2 38nm处出现吸收高峰。研究表明 ,铜绿微囊藻菌CCAP1450 / 4中铜绿微囊藻毒素的主要成份是铜绿微囊藻毒素LR。本研究探索了简便、快速人工培养铜绿微囊藻菌及高效、可靠分离纯化铜绿微囊藻毒素LR的方法。
Microcystis aruginosa CCAP 1450/4 was artificially cultured in a basal medium. It showed that greater surface area of medium, continuous light and optimum temperature enhanced the cell production. M.aeruginosa cell was broken up by ultrasonication. The toxic peptide were extracted by a series of isolation procedures including centrifugation, filtration and C 18 column elution. HPLC method were used to purify the peptide. The UV spectrum of the purified peak showed a peak of absorbance at 238 nm. It indicated that major component of microcystin from M.aeruginosa CCAP 1450/4 was microcystin LR. An efficient total system for M.aeruginosa cell culture and microcystin LR extraction were established.
基金
国家留学基金委员会资助项目(97814035)
山西省归国留学人员科研资助项目(20001087)
