摘要
对既杀鞘翅目昆虫又杀鳞翅目昆虫的广谱重组Bt菌株LCJ 0 8,LCJ 12进行了摇瓶发酵和中试发酵。通过 2 .4吨罐发酵试验 ,优化了发酵培养基和发酵条件 ,发酵效价达到 140 0 - 170 0IU/μL(棉铃虫 )和 2 0 0 0CU/μL(柳兰叶甲 )。以直接喷雾干燥和加填充料后喷雾干燥方法生产的粉剂 ,效价分别达到 2 50 0 0 - 34 0 0 0IU/mg和 82 0 0IU/mg。以低倍浓缩工艺生产的悬乳剂 ,毒力效价 2 50 0IU/μL以上。
Wide spectrum recombinants of bacillus thuringiensis LCJ 08 and LCJ 12 were tested in shake flask and 2.4 ton fermentor. Suspension emulsion of Bt recombinants had the lepidterancidal titer of 2500-2800IU/ul and the coleopterancidal titer of 2200-3000CU/ul. Wettable powder of Bt recombinants had the lepidopterancidal titer of 25000-34000IU/mg.
基金
国家"九五"科技攻关子专题!(96 C0 1 0 2 0 1)
