毫米波对人骨髓细胞和白血病细胞克隆能力的作用

Effects of Millimeter-wave Radiation on the Clonogenic Capacity of Human Bone Marrow Cell and Leukemic Cells

【目的】探索毫米波对人骨髓细胞和白血病细胞克隆能力的影响。【方法】以波长 8mm ,功率密度为 4mW /cm2的毫米波辐射人骨髓细胞和HL6 0细胞、K5 6 2细胞 ,测定粒巨噬细胞集落形成单位 (GM CFU)和肿瘤细胞集落形成单位 (tC FU)。【结果】连续 2次 6 0min的辐射使实验组的GM CFU比对照组明显上升 (P <0 0 5 ) ,连续 10次 30min或 6 0min的辐射均使tCFU较之于对照组显著降低 (P <0 0 5或P <0 0 1)。其余辐射方式则对GM CFU和tCFU的变化无明显作用 (P>0 0 5 )。【结论】毫米波的短期辐射 (2次 )能增强正常人骨髓细胞的克隆能力而不影响HL6 0细胞和K5 6 2细胞的克隆能力 ,较长时间的辐射 (10次 )则使HL6 0细胞及K5 6 2细胞的克隆能力明显降低。提示微波的非热效应可能会影响白血病细胞的净化。

Objective To study the effects of millimeter-wave radiation on the clonogenic capacity of human bone marrow cell and leukemic cells. Methods Normal human bone marrow cells、 HL60 and K562 were exposed to 8 0 millimeter-wave at a power density of 4 mW/cm 2. GM-CFU and tCFU were measured. Results It was found that two times of 60 minutes radiation could result in significant increase of GM-CFU (P<0 05) while ten times of 30 minutes or 60 minutes radiation could lead to remarkable decrease of tCFU compared with control group(P<0 05 or P<0 01). And the other kinds of radiation had no significant effects on both GM-CFU and tCFU(P>0 05). Conclusion A short-time millimeter-wave radiation (twice) could strengthen the clonogenic capacity of human bone marrow cell but not on that of HL60 cells and K562 cells. The longer time millimeter-wave radiation (ten times) could decreased the clonogenic capacity of HL60 cells and K562 cells significantly. It was suggested that nonthermal effect of microwave might influence purgation of leukemic cells.