亚氨基二乙酸的高效液相色谱测定

HPLC Determination of lminodiacetic Acid

本文描述利用液相色谱 ,采用外标法测定样品中亚氨基二乙酸的含量 ,紫外检测器检测波长为 2 1 0nm ,色谱柱为SAX强阴离子交换柱 ,采用 0 .0 0 5M磷酸二氢钾缓冲液 (pH =2 .5)为流动相 ,对样品进行分析。线性相关系数为 0 .9996,标准偏差为 0 .0 35,实验回收率为 98.5 %～ 1 0 1 .5%。

The determination of IDA by HPLC Was described.The external standard method was adopted,The chromatagraphic conditions included:UV detection at 210 nm,the column as SAX column,the mobile phase including 0.005M KH 2PO 4(pH=2.5),The Correlation coefficient is 0.9996,the standard deviation is 0.035,the recovery ration is 98.5 %～101.5 %.