水蛭对坐骨神经损伤再生作用影响的实验研究

The Effect of Leech on the Regeneration of the Injured Sciatic Nerve

目的:研究再生能力强的动物性中药水蛭对周围神经损伤再生作用的影响。方法:本实验选用健康清洁级SD雄性大鼠36只,随机分成水蛭组、对照组。2组大鼠均在右后肢梨状肌下缘0.5cm处用特制的无齿止血钳钳夹坐骨神经干,术后3d分别予以灌服相应的药物。用药后2、4、8周时进行坐骨神经功能指数测定、神经传导速度测定、小腿三头肌湿重测定,并进行组织学和超微结构电子显微镜观察,以示神经的再生情况。结果:水蛭组在神经功能指数、传导速度、小腿三头肌湿重及神经再生方面均明显优于对照组。结论:水蛭可有效促进大鼠损伤坐骨神经的再生。

Objective：To study the effect of leech on the regeneration of the injuried sciatic nerve and provide the theory basis for clinical application of peripheral nerve injury.Methods： Sixty male clean SD rats were randomly divided into leech group and control group.An injury of the right sciatic nerve under 0.5cm of musculus piriformis was made by specially made non-tooth artery forceps.Leech solution and normal saline were administered respectively every day,3 days after surgery.Sciatic nerve function index,sciatic nerve conduct velocity,tricipital muscle wet weight,histomorphological indexes and ultrastructure with the transmission election microscope were observed at the second,4th,8th week.Results：There were significant difference in all the indexes between the leech group and the control group.Conclusions：Leech may effectively promote the functional recovery of peripheral nerve injury and were deserved the further research.